Control solution was prepared similarly but by excluding lanosterol. Lanosterol 25 mM was prepared by adding double distilled H 2O to a mixture of 12.5 g lanosterol (Tokyo Chemical Industry, Japan) 1.1 g (EDTA) 2 Na, 0.55 g alkyl dimethyl benzyl ammonium chloride, and 200 ml EtOH to a final volume of 1.1 l. Parameters such as illumination, distance of the camera from the nucleus, magnification, and ISO were standardized for obtaining the images. The nuclei were then placed over a grid, illuminated from below, and photographed. The nuclei were immersed immediately in balanced salt solution and shaken to remove the adherent residual cortex. Diabetic and posttraumatic cataracts were excluded from the study. Forty cataractous nuclei of patients undergoing manual small incision cataract surgery for senile cataract were obtained for this study.
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